NKAP ELISA Kit

Produktnummer ABIN5674704

Produktdetails NKAP ELISA Kit

Target: NKAP (NFKB Activating Protein (NKAP))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.25 pg/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 11.3 pg/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu NKAP

Target: NKAP (NFKB Activating Protein (NKAP))

Abstract: NKAP Produkte

Synonyme: DSMA4 ELISA Kit, GEF720 ELISA Kit, Syx ELISA Kit, Tech ELISA Kit, c6orf194l ELISA Kit, wu:fj36d09 ELISA Kit, wu:fj49c09 ELISA Kit, 2610020O08Rik ELISA Kit, AA987160 ELISA Kit, AI849147 ELISA Kit, AL024236 ELISA Kit, 2610020o08rik ELISA Kit, nkap ELISA Kit, NKAPL ELISA Kit, NFKB activating protein ELISA Kit, pleckstrin homology and RhoGEF domain containing G5 ELISA Kit, NFKB activating protein S homeolog ELISA Kit, NKAP ELISA Kit, PLEKHG5 ELISA Kit, nkap ELISA Kit, Nkap ELISA Kit, nkap.S ELISA Kit

Hintergrund: Alternative name: NF Kappa B Activating Protein

Gen-ID: 67050

UniProt: Q9D0F4

Pathways: Stem Cell Maintenance, Phosphorylierungen bei SARS-CoV-2 Infektion