NCF1 ELISA Kit

Produktnummer ABIN2044091

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human NCF1.

Kurzübersicht für NCF1 ELISA Kit (ABIN2044091)

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails NCF1 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.117 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Neutrophil Cytosolic Factor 1 (NCF1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Cytosolic Factor 1 (NCF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neutrophil Cytosolic Factor 1 (NCF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutrophil Cytosolic Factor 1 (NCF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Neutrophil Cytosolic Factor 1 (NCF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Andere Bezeichnung: Neutrophil Cytosolic Factor 1 (NCF1)

Pathways: PI3K-Akt Signalweg