NAPSA ELISA Kit

Produktnummer ABIN2044049

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human NAPSA.

Kurzübersicht für NAPSA ELISA Kit (ABIN2044049)

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 46.87-3000 pg/mL

Applikation: ELISA

Produktdetails NAPSA ELISA Kit

Untere Nachweisgrenze: 46.87 pg/mL

Sensitivität: < 18.22 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Napsin A Aspartic Peptidase (NAPSA) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Napsin A Aspartic Peptidase (NAPSA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Napsin A Aspartic Peptidase (NAPSA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Napsin A Aspartic Peptidase (NAPSA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Napsin A Aspartic Peptidase (NAPSA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu NAPSA

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Pathways: Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis