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NAPSA ELISA Kit

NAPSA Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4883854
  • Target Alle NAPSA ELISA Kits anzeigen
    NAPSA (Napsin A Aspartic Peptidase (NAPSA))
    Reaktivität
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human Napsin A/NAPSA ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes .
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle NAPSA ELISA Kits anzeigen
    NAPSA (Napsin A Aspartic Peptidase (NAPSA))
    Andere Bezeichnung
    Napsin A (NAPSA Produkte)
    Synonyme
    KAP ELISA Kit, Kdap ELISA Kit, NAP1 ELISA Kit, NAPA ELISA Kit, SNAPA ELISA Kit, pronapsin ELISA Kit, kap ELISA Kit, kdap ELISA Kit, nap1 ELISA Kit, napa ELISA Kit, napsa ELISA Kit, snapa ELISA Kit, napsin A aspartic peptidase ELISA Kit, napsin A aspartic peptidase L homeolog ELISA Kit, NAPSA ELISA Kit, Napsa ELISA Kit, napsa.L ELISA Kit
    Gen-ID
    9476
    UniProt
    O96009
    Pathways
    Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis
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