MAP1LC3A ELISA Kit

Produktnummer ABIN2043973

Human MAP1LC3A ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human MAP1LC3A.

Kurzübersicht für MAP1LC3A ELISA Kit (ABIN2043973)

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails MAP1LC3A ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Sensitivität: < 0.59 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Andere Bezeichnung: Microtubule Associated Protein 1 Light Chain 3 alpha (MAP1LC3a)

Pathways: Autophagie