MMP2 ELISA Kit

Produktnummer ABIN2043943

Pferd MMP2 ELISA Kit, Colorimetric Assay für die Quantifizierung von Pferd MMP2.

Kurzübersicht für MMP2 ELISA Kit (ABIN2043943)

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Reaktivität: Pferd

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails MMP2 ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Sensitivität: < 0.61 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Matrix Metalloproteinase 2 (MMP2) BioAssay-ELISA Kit (Horse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 2 (MMP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 2 (MMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 2 (MMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Matrix Metalloproteinase 2 (MMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu MMP2

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Pathways: Activation of Innate immune Response