HLA-B ELISA Kit

Produktnummer ABIN2043915

Dieses Human HLA-B ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human HLA-B zu quantifizieren.

Kurzübersicht für HLA-B ELISA Kit (ABIN2043915)

Target: HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails HLA-B ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Marke: BioAssay™

Sensitivität: < 0.63 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Major Histocompatibility Complex Class I B (MHCB) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class I B (MHCB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class I B (MHCB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class I B (MHCB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Major Histocompatibility Complex Class I B (MHCB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu HLA-B

Target: HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))

Andere Bezeichnung: Major Histocompatibility Complex Class I B (MHCB)

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response