HLA-A ELISA Kit

Produktnummer ABIN2043913

Human HLA-A ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human HLA-A.

Kurzübersicht für HLA-A ELISA Kit (ABIN2043913)

Target: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails HLA-A ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.126 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Major Histocompatibility Complex Class I A (MHCA) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class I A (MHCA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class I A (MHCA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class I A (MHCA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Major Histocompatibility Complex Class I A (MHCA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu HLA-A

Target: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Andere Bezeichnung: Major Histocompatibility Complex Class I A (MHCA)

Pathways: T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response