LIN28A ELISA Kit

Produktnummer ABIN2043891

Das Maus LIN28A ELISA Kit (ABIN2043891) ist ein Colorimetric ELISA Kit zur Detektion von Maus LIN28A.

Kurzübersicht für LIN28A ELISA Kit (ABIN2043891)

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails LIN28A ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.049 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Lin-28 Homolog A (LIN28A) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lin-28 Homolog A (LIN28A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lin-28 Homolog A (LIN28A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lin-28 Homolog A (LIN28A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Lin-28 Homolog A (LIN28A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu LIN28A

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Andere Bezeichnung: Lin-28 Homolog A (LIN28A)

Pathways: Stem Cell Maintenance