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LIN28A ELISA Kit

LIN28A Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4883618
  • Target Alle LIN28A ELISA Kits anzeigen
    LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))
    Reaktivität
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human Lin-28A ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes .
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
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  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle LIN28A ELISA Kits anzeigen
    LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))
    Andere Bezeichnung
    Lin-28A (LIN28A Produkte)
    Synonyme
    CSDD1 ELISA Kit, LIN-28 ELISA Kit, LIN28 ELISA Kit, ZCCHC1 ELISA Kit, lin-28A ELISA Kit, AL024421 ELISA Kit, ENSMUSG00000070700 ELISA Kit, Gm10299 ELISA Kit, Lin-28 ELISA Kit, Lin28 ELISA Kit, Tex17 ELISA Kit, csdd1 ELISA Kit, lin-28 ELISA Kit, lin28 ELISA Kit, zcchc1 ELISA Kit, sb:cb650 ELISA Kit, zgc:55584 ELISA Kit, RGD1566408 ELISA Kit, lin-28 homolog A ELISA Kit, lin-28 homolog A (C. elegans) ELISA Kit, lin-28 homolog A S homeolog ELISA Kit, LIN28A ELISA Kit, Lin28a ELISA Kit, lin28a.S ELISA Kit, lin28a ELISA Kit
    Gen-ID
    79727
    UniProt
    Q9H9Z2
    Pathways
    Stem Cell Maintenance
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