Cytokeratin 18 ELISA Kit

Produktnummer ABIN2043851

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Schwein Cytokeratin 18.

Kurzübersicht für Cytokeratin 18 ELISA Kit (ABIN2043851)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reaktivität: Schwein

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails Cytokeratin 18 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.054 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Keratin 18 (KRT18) BioAssay-ELISA Kit (Porcine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 18 (KRT18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 18 (KRT18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 18 (KRT18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Keratin 18 (KRT18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Pathways: Apoptose, Caspase Kaskade in der Apoptose