IL-7 ELISA Kit

Produktnummer ABIN2043817

Maus IL-7 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus IL-7.

Kurzübersicht für IL-7 ELISA Kit (ABIN2043817)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62-1000 pg/mL

Applikation: ELISA

Produktdetails IL-7 ELISA Kit

Untere Nachweisgrenze: 15.62 pg/mL

Sensitivität: < 5.1 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Interleukin 7 (IL7) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 7 (IL7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 7 (IL7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 7 (IL7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 7 (IL7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Pathways: JAK-STAT Signalweg