IL-4 ELISA Kit

Produktnummer ABIN2043811

Affe IL-4 ELISA Kit, Colorimetric Assay für die Quantifizierung von Affe IL-4.

Kurzübersicht für IL-4 ELISA Kit (ABIN2043811)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reaktivität: Affe

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.12-200 pg/mL

Applikation: ELISA

Produktdetails IL-4 ELISA Kit

Untere Nachweisgrenze: 3.12 pg/mL

Sensitivität: < 1.26 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Interleukin 4 (IL4) BioAssay-ELISA Kit (Monkey) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 4 (IL4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 4 (IL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 4 (IL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 4 (IL4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation