IL-13 ELISA Kit

Produktnummer ABIN2043803

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Rind (Kuh) IL-13.

Kurzübersicht für IL-13 ELISA Kit (ABIN2043803)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Reaktivität: Rind (Kuh)

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.81-500 pg/mL

Applikation: ELISA

Produktdetails IL-13 ELISA Kit

Untere Nachweisgrenze: 7.81 pg/mL

Sensitivität: < 2.7 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Interleukin 13 (IL13) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 13 (IL13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 13 (IL13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 13 (IL13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 13 (IL13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Pathways: JAK-STAT Signalweg, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport