IL12B ELISA Kit

Produktnummer ABIN2043801

Dieses Schwein IL12B ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Schwein IL12B zu quantifizieren.

Kurzübersicht für IL12B ELISA Kit (ABIN2043801)

Target: IL12B (Interleukin 12 beta (IL12B))

Reaktivität: Schwein

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.81-500 pg/mL

Applikation: ELISA

Produktdetails IL12B ELISA Kit

Untere Nachweisgrenze: 7.81 pg/mL

Sensitivität: < 2.8 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Interleukin 12B (IL12B) BioAssay-ELISA Kit (Porcine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12B (IL12B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 12B (IL12B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12B (IL12B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 12B (IL12B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IL12B

Target: IL12B (Interleukin 12 beta (IL12B))

Andere Bezeichnung: Interleukin 12B (IL12B)

Pathways: JAK-STAT Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation