IFNA13 ELISA Kit

Produktnummer ABIN2043779

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Ratte IFNA13.

Kurzübersicht für IFNA13 ELISA Kit (ABIN2043779)

Target: IFNA13 (Interferon, alpha 13 (IFNA13))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.81-500 pg/mL

Applikation: ELISA

Produktdetails IFNA13 ELISA Kit

Untere Nachweisgrenze: 7.81 pg/mL

Sensitivität: < 2.8 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Interferon Alpha 13 (IFNa13) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha 13 (IFNa13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Alpha 13 (IFNa13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Alpha 13 (IFNa13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interferon Alpha 13 (IFNa13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IFNA13

Target: IFNA13 (Interferon, alpha 13 (IFNA13))

Andere Bezeichnung: Interferon alpha 13 (IFNa13)

Pathways: JAK-STAT Signalweg, Hepatitis C