ChT ELISA Kit

Produktnummer ABIN2043643

Ratte ChT ELISA Kit, Colorimetric Assay für die Quantifizierung von Ratte ChT.

Kurzübersicht für ChT ELISA Kit (ABIN2043643)

Target: ChT (High Affinity Choline Transporter (ChT))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12-5000 pg/mL

Applikation: ELISA

Produktdetails ChT ELISA Kit

Untere Nachweisgrenze: 78.12 pg/mL

Sensitivität: < 28 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: High Affinity Choline Transporter (ChT) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Affinity Choline Transporter (ChT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Affinity Choline Transporter (ChT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Affinity Choline Transporter (ChT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of High Affinity Choline Transporter (ChT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ChT

Target: ChT (High Affinity Choline Transporter (ChT))