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ChT ELISA Kit

ChT Reaktivität: Maus Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5674901
  • Target Alle ChT ELISA Kits anzeigen
    ChT (High Affinity Choline Transporter (ChT))
    Reaktivität
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Sensitivität
    0055 ng/mL
    Bestandteile
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Benötigtes Material
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    12 months
  • Target Alle ChT ELISA Kits anzeigen
    ChT (High Affinity Choline Transporter (ChT))
    Abstract
    ChT Produkte
    Synonyme
    CHT ELISA Kit, CHT1 ELISA Kit, HMN7A ELISA Kit, hCHT ELISA Kit, Cht1 ELISA Kit, solute carrier family 5 member 7 ELISA Kit, solute carrier family 5 (choline transporter), member 7 ELISA Kit, SLC5A7 ELISA Kit, Slc5a7 ELISA Kit
    Hintergrund
    Alternative name: SLC5A7, hCHT, CHT1, Solute Carrier Family 5 Member 7,Choline Transporter, Hemicholinium-3-sensitive choline transporter
    Gen-ID
    63993
    UniProt
    Q8BGY9
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