GADD45G ELISA Kit

Produktnummer ABIN2043601

Maus GADD45G ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus GADD45G.

Kurzübersicht für GADD45G ELISA Kit (ABIN2043601)

Target: GADD45G (Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.62-40 ng/mL

Applikation: ELISA

Produktdetails GADD45G ELISA Kit

Untere Nachweisgrenze: 0.62 ng/mL

Sensitivität: < 0.225 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu GADD45G

Target: GADD45G (Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G))

Andere Bezeichnung: Growth Arrest And DNA Damage Inducible Protein gamma (GADD45g)

Pathways: Zellzyklus