GADD45A ELISA Kit

Produktnummer ABIN2043599

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Ratte GADD45A.

Kurzübersicht für GADD45A ELISA Kit (ABIN2043599)

Target: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails GADD45A ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.062 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu GADD45A

Target: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Andere Bezeichnung: Growth Arrest And DNA Damage Inducible Protein alpha (GADD45a)

Pathways: p53 Signalweg, Zellzyklus