MBOAT4 ELISA Kit

Produktnummer ABIN2043541

Human MBOAT4 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human MBOAT4.

Kurzübersicht für MBOAT4 ELISA Kit (ABIN2043541)

Target: MBOAT4 (Membrane Bound O-Acyltransferase Domain Containing 4 (MBOAT4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78-50 ng/mL

Applikation: ELISA

Produktdetails MBOAT4 ELISA Kit

Untere Nachweisgrenze: 0.78 ng/mL

Sensitivität: < 0.26 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Ghrelin-O-Acyltransferase (GOAT) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ghrelin-O-Acyltransferase (GOAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ghrelin-O-Acyltransferase (GOAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ghrelin-O-Acyltransferase (GOAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ghrelin-O-Acyltransferase (GOAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu MBOAT4

Target: MBOAT4 (Membrane Bound O-Acyltransferase Domain Containing 4 (MBOAT4))

Andere Bezeichnung: Ghrelin-O-Acyltransferase (GOAT)

Pathways: Peptide Hormone Metabolism