FRZB ELISA Kit

Produktnummer ABIN2043511

Human FRZB ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human FRZB.

Kurzübersicht für FRZB ELISA Kit (ABIN2043511)

Target: FRZB (Frizzled-Related Protein (FRZB))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25-2000 pg/mL

Applikation: ELISA

Produktdetails FRZB ELISA Kit

Untere Nachweisgrenze: 31.25 pg/mL

Sensitivität: < 12.9 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Frizzled Related Protein (FRZB) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Frizzled Related Protein (FRZB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Frizzled Related Protein (FRZB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Frizzled Related Protein (FRZB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Frizzled Related Protein (FRZB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu FRZB

Target: FRZB (Frizzled-Related Protein (FRZB))

Andere Bezeichnung: Frizzled Related Protein (FRZB)

Pathways: WNT Signalweg, Positive Regulation of fat Cell Differentiation