FGF21 ELISA Kit

Produktnummer ABIN2043473

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus FGF21.

Kurzübersicht für FGF21 ELISA Kit (ABIN2043473)

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.81-500 pg/mL

Applikation: ELISA

Produktdetails FGF21 ELISA Kit

Untere Nachweisgrenze: 7.81 pg/mL

Sensitivität: < 3.2 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Fibroblast Growth Factor 21 (FGF21) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 21 (FGF21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 21 (FGF21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 21 (FGF21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Fibroblast Growth Factor 21 (FGF21) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu FGF21

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Pathways: RTK Signalweg