XPO1 ELISA Kit

Produktnummer ABIN2043429

Dieses Human XPO1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human XPO1 zu quantifizieren.

Kurzübersicht für XPO1 ELISA Kit (ABIN2043429)

Target: XPO1 (Exportin 1 (XPO1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails XPO1 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.123 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Exportin 1 (XPO1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Exportin 1 (XPO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Exportin 1 (XPO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Exportin 1 (XPO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Exportin 1 (XPO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu XPO1

Target: XPO1 (Exportin 1 (XPO1))

Pathways: M Phase