DLGAP5 ELISA Kit

Produktnummer ABIN2043353

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human DLGAP5.

Kurzübersicht für DLGAP5 ELISA Kit (ABIN2043353)

Target: DLGAP5 (Discs, Large (Drosophila) Homolog-Associated Protein 5 (DLGAP5))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails DLGAP5 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.113 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Discs, Large Homolog Associated Protein 5 (DLGAP5) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Discs, Large Homolog Associated Protein 5 (DLGAP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Discs, Large Homolog Associated Protein 5 (DLGAP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Discs, Large Homolog Associated Protein 5 (DLGAP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Discs, Large Homolog Associated Protein 5 (DLGAP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu DLGAP5

Target: DLGAP5 (Discs, Large (Drosophila) Homolog-Associated Protein 5 (DLGAP5))

Andere Bezeichnung: Discs, Large Homolog Associated Protein 5 (DLGAP5)

Pathways: M Phase