C3 ELISA Kit

Produktnummer ABIN2043197

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Huhn C3.

Kurzübersicht für C3 ELISA Kit (ABIN2043197)

Target: C3 (Complement Component 3 (C3))

Reaktivität: Huhn

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25-2000 ng/mL

Applikation: ELISA

Produktdetails C3 ELISA Kit

Untere Nachweisgrenze: 31.25 ng/mL

Sensitivität: < 11.2 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Complement Component 3 (C3) BioAssay-ELISA Kit (Chicken) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 3 (C3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 3 (C3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 3 (C3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Complement Component 3 (C3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu C3

Target: C3 (Complement Component 3 (C3))

Pathways: Komplementsystem, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling