C1QA ELISA Kit

Produktnummer ABIN2043195

Dieses Ratte C1QA ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Ratte C1QA zu quantifizieren.

Kurzübersicht für C1QA ELISA Kit (ABIN2043195)

Target: C1QA (Complement Component 1, Q Subcomponent, A Chain (C1QA))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails C1QA ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Sensitivität: < 0.72 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Complement Component 1, Q Subcomponent A (C1qA) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 1, Q Subcomponent A (C1qA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 1, Q Subcomponent A (C1qA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 1, Q Subcomponent A (C1qA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Complement Component 1, Q Subcomponent A (C1qA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu C1QA

Target: C1QA (Complement Component 1, Q Subcomponent, A Chain (C1QA))

Andere Bezeichnung: Complement Component 1, Q Subcomponent A (C1qA)

Pathways: Komplementsystem