Cathepsin S ELISA Kit

Produktnummer ABIN2043093

Dieses Ratte Cathepsin S ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Ratte Cathepsin S zu quantifizieren.

Kurzübersicht für Cathepsin S ELISA Kit (ABIN2043093)

Target: Cathepsin S (CTSS)

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.62-40 ng/mL

Applikation: ELISA

Produktdetails Cathepsin S ELISA Kit

Untere Nachweisgrenze: 0.62 ng/mL

Sensitivität: < 0.227 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Cathepsin S (CTSS) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin S (CTSS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin S (CTSS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin S (CTSS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cathepsin S (CTSS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Cathepsin S

Target: Cathepsin S (CTSS)

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades