HLAG ELISA Kit

Produktnummer ABIN2043001

Ratte HLAG ELISA Kit, Colorimetric Assay für die Quantifizierung von Ratte HLAG.

Kurzübersicht für HLAG ELISA Kit (ABIN2043001)

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails HLAG ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.136 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Beta-2-Microglobulin (b2M) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-2-Microglobulin (b2M). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta-2-Microglobulin (b2M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-2-Microglobulin (b2M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Beta-2-Microglobulin (b2M) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu HLAG

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Andere Bezeichnung: b2-Microglobulin (b2M)

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response