BIM ELISA Kit

Produktnummer ABIN2042999

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human BIM.

Kurzübersicht für BIM ELISA Kit (ABIN2042999)

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails BIM ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.114 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Bcl2 Like Protein 11 (BCL2L11) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Like Protein 11 (BCL2L11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2 Like Protein 11 (BCL2L11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Like Protein 11 (BCL2L11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Bcl2 Like Protein 11 (BCL2L11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu BIM

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Andere Bezeichnung: Bcl2 Like Protein 11 (BCL2L11)

Pathways: PI3K-Akt Signalweg, Neurotrophin Signalübertragung, Tube Formation, Positive Regulation of Endopeptidase Activity