BCL2L1 ELISA Kit

Produktnummer ABIN2042991

Dieses Maus BCL2L1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus BCL2L1 zu quantifizieren.

Kurzübersicht für BCL2L1 ELISA Kit (ABIN2042991)

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.81-500 pg/mL

Applikation: ELISA

Produktdetails BCL2L1 ELISA Kit

Untere Nachweisgrenze: 7.81 pg/mL

Sensitivität: < 3.3 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu BCL2L1

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Andere Bezeichnung: B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L)

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling