ATG7 ELISA Kit

Produktnummer ABIN2042983

Human ATG7 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human ATG7.

Kurzübersicht für ATG7 ELISA Kit (ABIN2042983)

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails ATG7 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.107 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Autophagy Related Protein 7 (ATG7) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autophagy Related Protein 7 (ATG7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Autophagy Related Protein 7 (ATG7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Autophagy Related Protein 7 (ATG7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Autophagy Related Protein 7 (ATG7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Andere Bezeichnung: Autophagy Related Protein 7 (ATG7)

Pathways: Response to Water Deprivation, Autophagie