ATG16L1 ELISA Kit

Produktnummer ABIN2042981

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human ATG16L1.

Kurzübersicht für ATG16L1 ELISA Kit (ABIN2042981)

Target: ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails ATG16L1 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.063 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Autophagy Related Protein 16 Like Protein 1 (ATG16L1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autophagy Related Protein 16 Like Protein 1 (ATG16L1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Autophagy Related Protein 16 Like Protein 1 (ATG16L1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Autophagy Related Protein 16 Like Protein 1 (ATG16L1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Autophagy Related Protein 16 Like Protein 1 (ATG16L1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ATG16L1

Target: ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))

Andere Bezeichnung: Autophagy Related Protein 16 Like Protein 1 (ATG16L1)

Pathways: Autophagie