APOA5 ELISA Kit

Produktnummer ABIN2042925

Dieses Ratte APOA5 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Ratte APOA5 zu quantifizieren.

Kurzübersicht für APOA5 ELISA Kit (ABIN2042925)

Target: APOA5 (Apolipoprotein A-V (APOA5))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails APOA5 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.059 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Apolipoprotein A5 (APOA5) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A5 (APOA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A5 (APOA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A5 (APOA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Apolipoprotein A5 (APOA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu APOA5

Target: APOA5 (Apolipoprotein A-V (APOA5))

Andere Bezeichnung: Apolipoprotein A5 (APOA5)

Pathways: Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism