APEX1 ELISA Kit

Produktnummer ABIN2042915

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus APEX1.

Kurzübersicht für APEX1 ELISA Kit (ABIN2042915)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails APEX1 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.051 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: APEX Nuclease 1 (APEX1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to APEX Nuclease 1 (APEX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to APEX Nuclease 1 (APEX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APEX Nuclease 1 (APEX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of APEX Nuclease 1 (APEX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Andere Bezeichnung: APEX Nuclease 1 (APEX1)

Pathways: DNA Reparatur, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus