Acetyl-CoA Carboxylase alpha ELISA Kit

Produktnummer ABIN2042755

Ratte Acetyl-CoA Carboxylase alpha ELISA-Kit Colorimetric-Assay zur Quantifizierung von Ratte Acetyl-CoA Carboxylase alpha.

Kurzübersicht für Acetyl-CoA Carboxylase alpha ELISA Kit (ABIN2042755)

Target: Acetyl-CoA Carboxylase alpha (ACACA)

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.12-200 ng/mL

Applikation: ELISA

Produktdetails Acetyl-CoA Carboxylase alpha ELISA Kit

Untere Nachweisgrenze: 3.12 ng/mL

Sensitivität: < 1.34 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Acetyl Coenzyme A Carboxylase Alpha (ACACa) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetyl Coenzyme A Carboxylase Alpha (ACACa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acetyl Coenzyme A Carboxylase Alpha (ACACa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetyl Coenzyme A Carboxylase Alpha (ACACa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Acetyl Coenzyme A Carboxylase Alpha (ACACa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Acetyl-CoA Carboxylase alpha

Target: Acetyl-CoA Carboxylase alpha (ACACA)

Andere Bezeichnung: Acetyl Coenzyme A Carboxylase alpha (ACACa)

Pathways: AMPK Signaling, Ribonucleoside Biosynthetic Process, Warburg Effekt