TEK ELISA Kit

Produktnummer ABIN2042679

Dieses Human TEK ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human TEK zu quantifizieren.

Kurzübersicht für TEK ELISA Kit (ABIN2042679)

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 6-10000 pg/mL

Applikation: ELISA

Produktdetails TEK ELISA Kit

Untere Nachweisgrenze: 6 pg/mL

Kreuzreaktivität (Details): There is cross-reactivates with human TIE1< 0.1 % .

Sensitivität: < 5 pg/mL

Bestandteile: 96-well plate pre-coated with anti-TIE2, Human
Lyophilized recombinant TIE2, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Human TIE2 in cell culture supernates, serum and plasma (heparin, EDTA).Tyrosine kinase with Ig and EGF homology domain 2 (Tie-2), also called TEK tyrosine kinase, endothelial (TEK). Tie-2 and tie-1 are expressed in early embryonic vascular system and in maternal decidual vascular endothelial cells, where the vasculature undergoes an active angiogenesis. Tie-2, but not tie-1, expression was also detected in extraembryonic mesoderm of the amnion. Angiogenesis is coordinated with follicular cell growth in goitrogenesis. The angiopoietins, Ang-1 and Ang-2, are angiogenic growth factors acting through Tie-2. Tie-2 and Ang-1 are expressed in thyroid epithelial and endothelial cells, and Tie-2 is regulated by TSH and cAMP in follicular cells. And Tie-2 expression is increased in goiter in both humans and rats, consistent with a role in goitrogenesis. Tie2/Ang-1 signaling pathway plays a critical role in the maintenance of HSCs in a quiescent state in the BM niche. And the Tie-2 signaling pathway is also critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu TEK

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Andere Bezeichnung: TIE2

Gen-ID: 7010

UniProt: Q02763

Pathways: RTK Signalweg, Growth Factor Binding