sL-Selectin ELISA Kit

Produktnummer ABIN2042666

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Ratte sL-Selectin.

Kurzübersicht für sL-Selectin ELISA Kit (ABIN2042666)

Target: sL-Selectin (Selectin L (Soluble) (sL-Selectin))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 5-40000 pg/mL

Applikation: ELISA

Produktdetails sL-Selectin ELISA Kit

Untere Nachweisgrenze: 5 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 20 pg/mL

Bestandteile: 96-well plate pre-coated with anti-sL-Selectin, Rat
Lyophilized recombinant sL-Selectin, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Rat sL-Selectin in cell culture supernates, serum and plasma (heparin, EDTA).L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1 L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu sL-Selectin

Target: sL-Selectin (Selectin L (Soluble) (sL-Selectin))

Gen-ID: 29259

UniProt: Q63762