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sL-Selectin ELISA Kit

sL-Selectin Reaktivität: Ratte Colorimetric Sandwich ELISA 625 pg/mL - 40000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Produktnummer ABIN6720287
  • Target Alle sL-Selectin Produkte
    sL-Selectin (Selectin L (Soluble) (sL-Selectin))
    Reaktivität
    • 2
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    625 pg/mL - 40000 pg/mL
    Untere Nachweisgrenze
    625 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich ELISA for Quantitative Detection of Antigen
    Proben
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytische Methode
    Quantitative
    Produktmerkmale

    Synonyms: A.11, AI528707, CD62 antigen ligand, CD62 antigen-like family member L, gp90 MEL, hLHRc, IgA nephropathy, susceptibility to, includedL Selectin, LAM1, LECAM1, Leu8, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu8, Leukocyte-endothelial cell adhesion molecule 1, LNHR, LSEL, Ly-22, Ly-m22, LYAM1, Lymph node homing receptor, Lymphocyte adhesion molecule 1, Lymphocyte antigen 22, Lymphocyte surface MEL-14 antigen, PLNHR, Selectin L, Selectin, lymphocyte, SELL, TQ1

    Background: L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL(L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1 L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

    Gene Name: SELL

    Production: Natural and recombinant rat sL-Selectin. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: W39-N332

  • Applikationshinweise

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 40000pg/mL, 20000pg/mL,10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL rat L-Selectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each rat L-Selectin standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 625pg/mL-40000pg/mL

    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    RT,4 °C,-20 °C
    Informationen zur Lagerung
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Target Alle sL-Selectin Produkte
    sL-Selectin (Selectin L (Soluble) (sL-Selectin))
    Andere Bezeichnung
    sL-Selectin (sL-Selectin Produkte)
    Gen-ID
    29259
    NCBI Accession
    NP_062050
    UniProt
    Q63762
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