sL-Selectin ELISA Kit

Produktnummer ABIN2042664

Dieses Human sL-Selectin ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human sL-Selectin zu quantifizieren.

Kurzübersicht für sL-Selectin ELISA Kit (ABIN2042664)

Target: sL-Selectin (Selectin L (Soluble) (sL-Selectin))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Produktdetails sL-Selectin ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Marke: BioAssay™

Analytische Methode: Quantitative

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 5 pg/mL

Bestandteile: 96-well plate pre-coated with anti-sL-Selectin, Human
Lyophilized recombinant sL-Selectin, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Human L-Selectin in cell culture supernates, serum and plasma (heparin, EDTA, citrate).L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL L-selectin is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu sL-Selectin

Target: sL-Selectin (Selectin L (Soluble) (sL-Selectin))

Gen-ID: 6402

UniProt: P14151