CCL2 ELISA Kit

Produktnummer ABIN2042587

Maus CCL2 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus CCL2.

Kurzübersicht für CCL2 ELISA Kit (ABIN2042587)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Applikation: ELISA

Produktdetails CCL2 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 6 pg/mL

Bestandteile: 96-well plate pre-coated with anti-MCP-1, Mouse
Lyophilized recombinant MCP-1, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Mouse MCP-1 in cell culture supernates and serum.Monocyte chemotactic protein-1 (MCP-1) is a member of the small inducible gene (SIG) family. It has been shown to play a role in the recruitment of monocytes to sites of injury and infection. By analysis of a panel of somatic cell hybrids, we have localized the MCP-1 gene, designated SCYA2, to human chromosome 17. In situ hybridization confirmed this assignment and further localized the gene to 17q11.2-q21.1.1 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.2 Monocyte chemoattractant protein-1 (MCP-1), regulated on activation, normal T cell expressed and secreted,and stimulation of monocytes from healthy carriers of the genotype GG with Mycobacterium tuberculosis antigens yielded higher MCP-1.3 The standard used in this kit is recombinant mouse MCP-1(Q24-R96) with the molecular mass of 8.5kD.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Andere Bezeichnung: MCP-1

Gen-ID: 20296

UniProt: P10148

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylierungen bei SARS-CoV-2 Infektion