IL-13 ELISA Kit

Produktnummer ABIN2042524

Maus IL-13 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus IL-13.

Kurzübersicht für IL-13 ELISA Kit (ABIN2042524)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Applikation: ELISA

Produktdetails IL-13 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 2 pg/mL

Bestandteile: 96-well plate pre-coated with anti-IL-13, Mouse
Lyophilized recombinant IL-13, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Mouse IL-13 in cell culture supernates, serum and plasma (heparin, EDTA).Interleukin (IL)-13 is a major inducer of fibrosis in many chronic infectious and autoimmune diseases.1 Recombinant IL-13 protein inhibits inflammatory cytokine production induced by lipopolysaccharide in human peripheral blood monocytes. Moreover, it synergizes with IL-13 in regulating interferon-gamma synthesis in large granular lymphocytes. Interleukin-13 may be critical in regulating inflammatory and immune responses.2 IL-13 and IL-4 are two cytokines produced by T helper type 2 cells, mast cells, and basophils. In addition to their physiological roles, these cytokines are also implicated in pathological conditions such as asthma and allergy.3 The genes encoding human IL-4 and IL-13 are located on segment q23-31 of chromosome 5.4 Recombinant mouse IL-13 is a mixture of Ser26-Phe131 amino acids with the molecular mass of 11.5kD.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Andere Bezeichnung: IL-13

Gen-ID: 16163

UniProt: P20109

Pathways: JAK-STAT Signalweg, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport