IL-10 ELISA Kit

Produktnummer ABIN2042516

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human IL-10.

Kurzübersicht für IL-10 ELISA Kit (ABIN2042516)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8-500 pg/mL

Applikation: ELISA

Produktdetails IL-10 ELISA Kit

Untere Nachweisgrenze: 7.8 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 0.5 pg/mL

Produktmerkmale: Specific detection range: 7.8pg/mL-500pg/mL(body fluids, tissue lysates or cell culture supernates)

Bestandteile: 96-well plate pre-coated with anti-IL-10, Human
Lyophilized recombinant IL-10, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Human IL-10 in cell culture supernates, serum and plasma (heparin, EDTA, citrate.Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production.1 IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions.2 It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system.3 IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8.4 Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility.5 With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease.6 The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.7 The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6kD.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Andere Bezeichnung: IL-10

Gen-ID: 3586

UniProt: P22301

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints