HSP27 ELISA Kit

Produktnummer ABIN2042492

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human HSP27.

Kurzübersicht für HSP27 ELISA Kit (ABIN2042492)

Target: HSP27 (Heat Shock Protein 27 (HSP27))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Produktdetails HSP27 ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 5 pg/mL

Bestandteile: 96-well plate pre-coated with anti-HSP27, Human
Lyophilized recombinant HSP27, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Human HSP27 in cell lysates.Hsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, -crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. The HSP27 gene has 3 exons. The mouse Hsp25 gene was mapped to chromosome 5 in a region homologous to 7q in the human. They also mapped the mouse Hsp105 gene to chromosome 5 but suggested that the human homolog is probably on 13q, not chromosome 7. The standard used in this kit is recombinant human HSP27, consisting of 205 amino acids with the molecular mass of 22.7kD.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu HSP27

Target: HSP27 (Heat Shock Protein 27 (HSP27))

Gen-ID: 3315

UniProt: P04792

Pathways: VEGF Signaling