CD25 ELISA Kit

Produktnummer ABIN2042394

Dieses Maus CD25 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus CD25 zu quantifizieren.

Kurzübersicht für CD25 ELISA Kit (ABIN2042394)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 8-5000 pg/mL

Applikation: ELISA

Produktdetails CD25 ELISA Kit

Untere Nachweisgrenze: 8 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 5 pg/mL

Bestandteile: 96-well plate pre-coated with anti-CD25/IL-2sR alpha, Mouse
Lyophilized recombinant CD25/IL-2sR alpha, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Mouse CD25/IL-2sR alpha in cell culture supernates, serum and plasma (heparin, EDTA).CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Andere Bezeichnung: CD25/IL-2sR alpha

Gen-ID: 16184

UniProt: P01590

Pathways: JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation