Cathepsin B ELISA Kit

Produktnummer ABIN2042371

Maus Cathepsin B ELISA Kit, Colorimetric Assay für die Quantifizierung von Maus Cathepsin B.

Kurzübersicht für Cathepsin B ELISA Kit (ABIN2042371)

Target: Cathepsin B (CTSB)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 6-10000 pg/mL

Applikation: ELISA

Produktdetails Cathepsin B ELISA Kit

Untere Nachweisgrenze: 6 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 5 pg/mL

Bestandteile: 96-well plate pre-coated with anti-Cathepsin B, Mouse
Lyophilized recombinant Cathepsin B, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Mouse Cathepsin B in cell culture supernates, serum and plasma (heparin, EDTA).Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of -amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade -amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant mouse Cathepsin B with the molecular mass of 37kD.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Cathepsin B

Target: Cathepsin B (CTSB)

Gen-ID: 13030

UniProt: P10605

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades