Angiopoietin 2 ELISA Kit

Produktnummer ABIN2042346

Dieses Human Angiopoietin 2 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human Angiopoietin 2 zu quantifizieren.

Kurzübersicht für Angiopoietin 2 ELISA Kit (ABIN2042346)

Target: Angiopoietin 2 (ANGPT2)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 6-10000 pg/mL

Applikation: ELISA

Produktdetails Angiopoietin 2 ELISA Kit

Untere Nachweisgrenze: 6 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile: 96-well plate pre-coated with anti-Angiopoietin-2, Human
Lyophilized recombinant Angiopoietin-2, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Human Angiopoietin-2 in cell culture supernates, serum, plasma (heparin, EDTA), saliva and human milk.Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin-2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation. Angiopoietin 1 binding to Tie 2 causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodelling dorsal aorta and major aortic branches, ovary, placenta and uterus.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Angiopoietin 2

Target: Angiopoietin 2 (ANGPT2)

Andere Bezeichnung: Angiopoietin-2

Gen-ID: 285

UniProt: O15123

Pathways: RTK Signalweg