Angiotensin I Converting Enzyme 1 ELISA Kit

Produktnummer ABIN2042334

Dieses Human Angiotensin I Converting Enzyme 1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human Angiotensin I Converting Enzyme 1 zu quantifizieren.

Kurzübersicht für Angiotensin I Converting Enzyme 1 ELISA Kit (ABIN2042334)

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 6-10000 pg/mL

Applikation: ELISA

Produktdetails Angiotensin I Converting Enzyme 1 ELISA Kit

Untere Nachweisgrenze: 6 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 5 pg/mL

Bestandteile: 96-well plate pre-coated with anti-ACE, Human
Lyophilized recombinant ACE, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Human ACE in cell culture supernates, serum, plasma (heparin) and saliva.Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073.1 Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm.2 The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120kD.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Angiotensin I Converting Enzyme 1

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Andere Bezeichnung: ACE

Gen-ID: 1636

UniProt: B4DKH4

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration