CAPN10 ELISA Kit

Produktnummer ABIN7540316

Produktdetails CAPN10 ELISA Kit

Target: CAPN10 (Calpain 10 (CAPN10))

Reaktivität: Chemical

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 1-243 pM/mL

Untere Nachweisgrenze: 1 pM/mL

Applikation: ELISA

Verwendungszweck: Quantitative detection of cAMP in samples such as serum, plasma, saliva, cell culture supernatant, and urine.

Proben: Serum, Plasma, Saliva, Cell Culture Supernatant, Urine

Analytische Methode: Quantitative

Spezifität: Easy - use: All reagents and buffers for cAMP test are provided.

Kreuzreaktivität (Details): No significant cross-reactivity of similar compounds was found

Sensitivität: 0.61 pmol/mL

Produktmerkmale: cAMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cAMP (Adenosine 3',5'-cyclic monophosphate) in samples such as serum, plasma, saliva, cell culture supernatant, and urine. cAMP is an important secondary messenger in signal transduction pathways that follows a number of extracellular signals. cAMP activates or inhibits various enzymes by promoting their phosphorylation or dephosphorylation. Its concentration is converted from adenosine triphosphate (ATP) via adenylyl cyclases (AC), and is inactivated by hydrolysis to 5'-AMP by the actions of phosphodiesterases. The anti-IgG Capture Plate is pre-coated with a fixed amount of Goat anti-mouse IgG to capture Mouse Anti-cAMP Monoclonal Antibody. When free cAMP or specimen and HRP-cAMP are added to the well, they compete in the solution to interact with the cAMP antibody captured on the plate. Other unbound molecules are removed by the wash step. The cAMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the cAMP amount present in the unknown samples can be calculated by transforming its absorbance value.

Bestandteile: Anti-IgG Capture Plate: 1 plate (8 wells x 12 strips)
Anti-cAMP mAb: 12 ml
HRP-cAMP: 6 ml
cAMP Standards (0, 1, 3, 9, 27, 81, 243 pmol/ml): 1.5 ml
cAMP Standard Stock (10 nmol/ml): 500 μl
Assay Buffer A: 60 ml
Assay Buffer B: 1 ml
20 × Wash Solution: 40 ml
TMB Substrate: 12 ml
Stop Solution: 6 ml
Plate Sealer: 2 pieces
User Manual: 1 copy

Anwendungsinformationen

Testdauer: 3.5 h

Plattentyp: Pre-coated

Protokoll: The anti-IgG Capture Plate is pre-coated with a fixed amount of Goat anti-mouse IgG to capture Mouse Anti-cAMP Monoclonal Antibody. When free cAMP or specimen and HRP-cAMP are added to the well, they compete in the solution to interact with the cAMP antibody captured on the plate. Other unbound molecules are removed by the wash step. The cAMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the cAMP amount present in the unknown samples can be calculated by transforming its absorbance value.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not freeze the kit.

Lagerung: 4 °C

Informationen zur Lagerung: The unopened kit is stable for at least 12 months if stored at 2-8 °C, and the opened kit is stable for up to 2 weeks at 2-8 °C.

Haltbarkeit: 12 months

Details zu CAPN10

Target: CAPN10 (Calpain 10 (CAPN10))

Andere Bezeichnung: cAMP / Cyclic AMP (CAPN10 Produkte)

Substanzklasse: Chemical

Hintergrund: CAMP is an important secondary messenger in signal transduction pathways that follows a number of extracellular signals. cAMP activates or inhibits various enzymes by promoting their phosphorylation or dephosphorylation. Its concentration is converted from adenosine triphosphate (ATP) via adenylyl cyclases (AC), and is inactivated by hydrolysis to 5'-AMP by the actions of phosphodiesterases.

Pathways: Positive Regulation of Peptide Hormone Secretion