FGF1 ELISA Kit

Produktnummer ABIN1979365

Produktdetails FGF1 ELISA Kit

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 12-4000 pg/mL

Untere Nachweisgrenze: 12 pg/mL

Applikation: ELISA

Verwendungszweck: Human aFGF (FGF-1) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA kit shows no cross-reactivity with the following cytokines tested: human BLC, BDNF, bFGF, BMP-4, BMP-5, BMP-7, b-NGF, Eotaxin-1, Eotaxin-2, FGF-4, FGF-7, G-CSF, GM-CSF, I-309, ICAM-1, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 ra, IL-2, IL-4, IL-5, IL-6, IL-6 sR, IL-7, IL-8, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-16, IL-17, MCP-1, M-CSF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PDGF-BB, RANTES, sTNFRI, TIMP-1, TIMP-2, TNF alpha, TNF beta, VEGF, VEGFR2, VEGFR3.

Kreuzreaktivität (Details): This ELISA kit shows no cross-reactivity with the following cytokines tested: human BLC, BDNF, bFGF, BMP-4, BMP-5, BMP-7, b-NGF, Eotaxin-1, Eotaxin-2, FGF-4, FGF-7, G-CSF, GM-CSF, I- 309, ICAM-1, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 ra, IL-2, IL-4, IL-5, IL-6, IL-6 sR, IL-7, IL-8, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-16, IL-17, MCP-1, M-CSF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PDGF-BB, RANTES, sTNFRI, TIMP-1, TIMP-2, TNF alpha, TNF beta, VEGF, VEGFR2, VEGFR3.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Aufbereitung der Reagenzien:

1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. 2. Sample dilution: If your samples need to be diluted, Assay Diluent (Item E) should be used for dilution of serum/plasms/cell culture supernatants/urine. Suggested dilution for normal serum/plasma: 2 fold. The Human aFGF ELISA Kit Protocol 3 Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator. 3. Assay Diluent (Item E) should be diluted 5-fold with deionized or distilled water before use. 4. Preparation of standard: Briefly spin the vial of Item C. Add 400 µL 1x Assay Diluent (Item E) into Item C vial to prepare a 50 ng/ml standard solution. Dissolve the powder thoroughly by a gentle mix. Add 40 µL of the aFGF standard from the vial of Item C, into a tube with 460 µL 1x Assay Diluent to prepare a 4,000 pg/ml standard solution. Pipette 300 µL 1x Assay Diluent into each tube. Use the 4,000 pg/ml standard solution to produce a dilution series (shown below). Mix each tube thoroughly before the next transfer. 1x Assay Diluent serves as the zero standard (0 pg/ml). 200 µL 40 µL standard + 460 µL 200myl 200 µL 200 µL 200 µL 200 µL 4,000 1,600 640 256 102.4 40.96 16.38 0 pg/ml pg/ml pg/ml pg/ml pg/ml pg/ml pg/ml pg/ml 5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 mL of Wash Buffer Concentrate into deionized or distilled water to yield 400 mL of 1x Wash Buffer. 6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent into the vial to prepare a detection The Human aFGF ELISA Kit Protocol 4 antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent and used in step 4 of Part VI Assay Procedure. 7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 500-fold with 1x Assay Diluent. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 20 µL of HRP-Streptavidin concentrate into a tube with 10 mL 1x Assay Diluent to prepare a 500-fold diluted HRP-Streptavidin solution (don't store the diluted solution for next day use). Mix well.

Testdurchführung:

1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate. 2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or overnight at 4 °C with gentle shaking. 3. Discard 4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking. 5. Discard the solution. Repeat the wash as in step 3. 6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking. 7. Discard the solution. Repeat the wash as in step 3. 8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking. 9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.

Ergebnisberechnung:

Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.

Testpräzision: Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu FGF1

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Andere Bezeichnung: aFGF (FGF1 Produkte)

Synonyme: AFGF ELISA Kit, ECGF ELISA Kit, ECGF-beta ELISA Kit, ECGFA ELISA Kit, ECGFB ELISA Kit, FGF-1 ELISA Kit, FGF-alpha ELISA Kit, FGFA ELISA Kit, GLIO703 ELISA Kit, HBGF-1 ELISA Kit, HBGF1 ELISA Kit, Fgf1 ELISA Kit, FGF1 ELISA Kit, aFGF ELISA Kit, ecgf ELISA Kit, fgfa ELISA Kit, ecgfa ELISA Kit, ecgfb ELISA Kit, fgf-1 ELISA Kit, hbgf1 ELISA Kit, glio703 ELISA Kit, ecgf-beta ELISA Kit, fgf-alpha ELISA Kit, Dffrx ELISA Kit, Fam ELISA Kit, Fgf-1 ELISA Kit, Fgfa ELISA Kit, zgc:136885 ELISA Kit, LOC100221393 ELISA Kit, fgf1 ELISA Kit, zgc:73249 ELISA Kit, fibroblast growth factor 1 ELISA Kit, fibroblast growth factor 1 L homeolog ELISA Kit, fibroblast growth factor 1b ELISA Kit, fibroblast growth factor 1a ELISA Kit, FGF1 ELISA Kit, fgf1.L ELISA Kit, fgf1 ELISA Kit, Fgf1 ELISA Kit, fgf1b ELISA Kit, LOC100221393 ELISA Kit, fgf1a ELISA Kit

Gen-ID: 2246

UniProt: P05230

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung